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Tuesday, December 16, 2025 at 5:22 AM

Forage Analysis: What Numbers Do I Need?

Post Rock Extension District

K-State Research and Extension-Post Rock Distric Livestock Production Agent

One of the more common questions regarding analytical testing of forages and other feedstuffs is “I have the sample, now what do I test for or what analysis package should I select?” The basic components that nutritionists need to evaluate a feedstuff or develop a ration are dry matter or moisture, crude protein, an estimate of the energy content of the feedstuff (Total Digestible Nutrients (TDN), Net Energy for Maintenance (NEm), Net Energy for gain (NEg), and the macro minerals, Calcium and Phosphorous. These are the most basic numbers that are required but including some additional analyses in the report can give us insight into the quality of the feedstuff. It can also improve our ability to predict animal performance which is the primary reason we analyze feedstuffs. It is recommended that the report include acid detergent fiber (ADF) and neutral detergent fiber (NDF). The amount of NDF in forage reflects the amount of cell wall contents (hemicellulose, cellulose, and lignin) within the sample. The NDF fraction is often associated with the respective bulkiness of forage and is correlated with dry matter intake of the forage or feedstuff. Therefore, the amount of NDF may be used to estimate the expected dry matter intake associated with the forage. The ADF number represents the amount of cellulose and lignin within the forage and is correlated with the respective digestibility of the forage. In general, a higher ADF value is associated with forage that has a greater proportion cellulose and lignin and would likely be more mature. Additionally, the ADF fraction is used to calculate the energy estimates TDN, NEm, and NEg that appear on the report. There are several different mathematical equations that the testing laboratory may use to calculate these numbers, based on the type of sample (corn silage, alfalfa, grass hay, etc.). If the ADF is included in the report, the nutritionist can adjust or recalculate the energy estimates if necessary.

If the forage is fed in combination with a byproduct feed such as wet distiller’s grain, including an analysis for sulfur can be beneficial if the forage will be used in a growing or feedlot ration. Additionally, if the forage is a known nitrate accumulator (forage sorghums, Sudan grass) or may have been stressed due to drought, including a nitrate analysis should always be considered, especially if the forage will be fed to pregnant cows.

Most analytical laboratories have many analysis packages which encompass the most common procedures or numbers that a nutritionist or producer needs to know about their feeds. These packages will typically include the basic procedures (DM, CP, TDN) and then add on specific analyses such as NDF, or the Macrominerals (Ca, P, Mg, K, Na, Cl, S). Some laboratories may group analysis packages by the type of sample (Forage, vs. mixed ration) or production purposes (dairy vs. beef).

The objective of analytical testing of forages and feedstuffs is to improve our ability to meet the animal’s nutrient requirements and ultimately predict animal performance. The unequivocal best method of evaluating the quality of a feedstuff is feeding it to an animal and evaluating performance over a set period, under a specific set of conditions. Since that would not be cost effective or timely, analytically evaluating feedstuffs in a laboratory is the next best thing and although it is not perfect, it is better than the “this looks like really good stuff” method of evaluating feedstuffs.

If you have further questions on forage testing, contact Blaire at any Post Rock Extension District Office in Beloit, Lincoln, Mankato, Osborne, or Smith Center.

Post Rock Extension District of K-State Research and Extension serves Jewell, Lincoln, Mitchell, Osborne, and Smith counties. Blaire may be contacted at [email protected] or by calling Beloit 7383597, Smith Center, 2826823, Lincoln 524-4432, Mankato 378-3174, or Osborne 346-2521. Join us on Facebook at “Post Rock Extension” and remember our website is www. postrock.ksu.edu.


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